Sxl Physical Interaction report

western blot

Collection

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

Sxl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Walthall et al., 2007)

FBrf0210202-10.coIP.PCR

Reference

Johnson et al., 2010

Description

physical association

Assay

reverse transcription pcr

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

Sxl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Johnson et al., 2010)

FBrf0214597-2.coIP.PCR

Reference

Graham et al., 2011

Description

physical association

Assay

Collection

Source/Stage

testis / male adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sxl

sxl

bait

Sxl

sxl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult males testes; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Graham et al., 2011)

FBrf0229644-4.coIP.PCR

Reference

Yan and Perrimon, 2015

Description

physical association

Assay

primer specific pcr

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

GFP tag

Sxl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yan and Perrimon, 2015)

FBrf0234210-1.coIP.PCR

Reference

Haussmann et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-ATCC

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

HA tag

Sxl

prey

Sxl

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Sxl

Sxl-regulated intron

sufficient binding region

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Haussmann et al., 2016)

FBrf0236006-8.coIP.PCR

Reference

Kan et al., 2017

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

GFP tag

Sxl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kan et al., 2017)

FBrf0241006-5.coIP.PCR

Reference

Sandler et al., 2018

Description

physical association

Assay

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

Sxl

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of wild-type flies; bait produced from endogenous gene; prey produced from endogenous gene.

(Sandler et al., 2018)

Positive control.

(Sandler et al., 2018)

FBrf0256719-2.coIP.PCR

Reference

Fan et al., 2023

Description

physical association

Assay

reverse transcription pcr

Collection

Source/Stage

Cell line used

S2-unspecified

Participants

Corresponds to

Reported as

Role

Note

Sxl

bait

Sxl

prey

Sxl

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Sxl

RNA binding residues

mutation decreasing interaction

aa 122, 123, 126, 244, 246, 248

N122A, Y123A, Q126A, R244A, V246A, F248A

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Fan et al., 2023)

FBrf0256719-3.coIP.SEQ

Reference

Fan et al., 2023

Description

physical association

Assay