Source was intact S2 cells; bait produced from transfected construct; prey produced from transfected construct.

The N-Dl interaction requires Ca[2+].

S2 cells expressing N were mixed with S2 cells expressing Dl, and the formation of cell clusters containing both N and Dl expressing cells was assayed by immunofluorescence.

Source was intact S2 cells; bait produced from transfected construct; prey produced from transfected construct.

The N-Dl interaction requires Ca[2+].

S2 cells expressing N were mixed with S2 cells expressing Dl, and the formation of cell clusters containing both N and Dl expressing cells was assayed by immunofluorescence.

Full-length N interacts with Dl in 0-3 hr embryos.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction in vitro; bait produced from transfected construct; prey produced from conditioned medium of stably transfected cell line.

Binding of Dl to N was greatly increased by cotransfection of N with fng.

S2 cells expressing N were incubated with Dl-conditioned medium, then analyzed for surface-bound alkaline phosphatase activity.

Interaction in vitro; bait produced from transfected construct; prey produced from conditioned medium of stably transfected cell line.

Binding of N to Dl was greatly increased by cotransfection of N with fng during production of conditioned medium.

S2 cells expressing Dl were incubated with N-conditioned medium, then analyzed for surface-bound alkaline phosphatase activity.

Source was live S2 cells; proteins produced from transfected constructs.

N did not interact with itself in this experiment.

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Interaction in vitro; bait and prey produced as a recombinant fusion proteins in S2 cells

Binding of N to Dl is increased in the presence of expressed fng.

ligand expressed on the surface of S2 cells (bait), AP tagged N purified from transfected S2 cells (prey).

Binding of N to Dl is increased in the presence of expressed fng.

Interaction in vitro; bait purified from media of transfected S2 cells; prey purified from media of transfected S2 cells.

The N-Dl interaction is stronger at pH 5.4 than at pH 7.4.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Binding of N to Dl is slightly increased by the N V361M mutation, which disrupts the N-Ser interaction.

Alkaline-phosphatase-tagged Dl was collected from conditioned media and mixed with S2 cells expressing N. After extensive washing of cells, the amount of alkaline phosphatase bound to cells was measured.

Source was live S2 cells; bait produced from transfected construct; prey purified from medium of transfected cells.

Co-expression of fng with N enhanced Dl binding; fng glycosyltransferase activity was required for enhancement; mutation of N O-fucosylation sites, T348V, T386V, S502A, decrease fng mediated enhancement of N-Dl binding.

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells. Co-expression of fng enhanced N-Dl binding; mutation of N O-fucosylation sites, T348V and S502A, together abolishes fng mediated enhancement of N-Dl binding.