Source was embryonic nuclear extract wild-type fly line; proteins produced from endogenous genes.

The complex of Su(z)12, E(z), Rpd3, esc and Caf1 has an apparent size of 600-650 kDa (gel filtration).

A histone methyltransferase activity was purified from embryonic nuclear extracts.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The 600 kDa PRC2 complex (E(z), esc, Su(z)12, Caf1) is also present in larger 1 MDa complexes that additionally contain Rpd3 and Pcl. The larger 1 MDa PRC2 complex is only observed in 0-16 hr embryos, not in 18-24 hr embryos.

Immunoprecipitation of Su(z)12 from size fractionated extracts containing complexes of about 1 MDa in size.

Source larval brain ring gland complexes; bait and prey produced from endogenous gene.

Fig5C

FigS13A

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FigS13C

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was cell extract of S2 cell line; Source was cell extract of S2 cell line; bait and prey produced from endogenous gene.

FigS13E