FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sd, vg
FlyBase ID
FBig0000097294
Interaction Network
Interactions Browser links
sd network
vg network
Reported Interactions
FBrf0105294-1.Y2H
Reference
Paumard-Rigal et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

fused to LexA DNA-binding domain

sd 
vg 

vg

prey

fused to B42 activation domain

vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
C-terminal region
sufficient binding region
aa 212-440

coordinates relative to sd-PB

vg 
C-terminal region
sufficient binding region
aa 276-453

coordinates relative to vg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Paumard-Rigal et al., 1998)

Two-hybrid system: yeast LexA-BD/B42-AD

(Paumard-Rigal et al., 1998)
FBrf0105934-1.PD.A
Reference
Simmonds et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

His tag

vg 

Vg

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Simmonds et al., 1998)
FBrf0105934-2.PD.A
Reference
Simmonds et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vg 

Vg

bait

His tag

vg 
sd 

Sd

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vg 
Sd-interaction domain
sufficient binding region
aa 279-335

coordinates relative to vg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Simmonds et al., 1998)
FBrf0105934-4.FW.A
Reference
Simmonds et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

His tag

sd 
vg 

Vg

prey

[35]S label

vg 
vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vg 
Sd-interaction domain
sufficient binding region
aa 279-335

coordinates relative to vg-PA

vg 
Sd-interaction domain
necessary binding region
aa 281-335

coordinates relative to vg-PA

sd 
C-terminal region
sufficient binding region
aa 240-440

coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.

(Simmonds et al., 1998)
FBrf0105934-5.FW.A
Reference
Simmonds et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vg 

Vg

bait

His tag

sd 

Sd

prey

[35]S label

sd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
C-terminal region
sufficient binding region
aa 240-440

coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.

(Simmonds et al., 1998)
FBrf0105934-6.Y2H
Reference
Simmonds et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vg 

Vg

bait

fused to GAL4 DNA-binding domain

sd 

Sd

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Simmonds et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Simmonds et al., 1998)
FBrf0105934-7.Y2H
Reference
Simmonds et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

fused to GAL4 DNA-binding domain

vg 

vg

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Simmonds et al., 1998)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Simmonds et al., 1998)
FBrf0138372-1.EMSA.A
Reference
Halder and Carroll, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vg 

Vg

neutral component

His tag

vg 

Vg

neutral component

His tag

vg 

Vg

neutral component

His tag

sd 

Sd

neutral component

His tag

sd 

Sd

neutral component

His tag

sd 

Sd

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Halder and Carroll, 2001)

Only heterodimers of vg and sd bind strongly to the DNA probes tested; alone, neither binds the DNA probes tested.

(Halder and Carroll, 2001)

vg and/or sd proteins were mixed with [32]P labeled DNA (ct, kni, salm probes tested). DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

(Halder and Carroll, 2001)
FBrf0138372-2.coIP.A
Reference
Halder and Carroll, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

Myc tag

vg 

Vg

prey
vg 
vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vg 
SID domain
sufficient binding region
aa 281-345

coordinates relative to vg-PA

vg 
SID domain
necessary binding region
aa 302-337

coordinates relative to vg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Halder and Carroll, 2001)
FBrf0174698-1.PD.A
Reference
Srivastava et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

SD

bait

GST tag

vg 

VG

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Srivastava et al., 2004)
FBrf0207115-1.coIP.WB
Reference
Deng et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

FLAG tag

vg 

vg

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct

(Deng et al., 2009)
FBrf0207115-3.PD.A
Reference
Deng et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

GST tag

vg 

vg

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Deng et al., 2009)
FBrf0221209-3.coIP.WB
Reference
Cagliero et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

SD

bait

FLAG tag

vg 

VG

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cagliero et al., 2013)

vg outcompetes yki for binding to sd when the three proteins are coexpressed.

(Cagliero et al., 2013)
FBrf0229775-10.coIP.WB
Reference
Li et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HSV tag

vg 

Vg

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Increased expression of yki has no effect on binding between sd and vg.

(Li et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2015)
FBrf0229775-2.coIP.WB
Reference
Li et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

FLAG tag

vg 

VG

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2015)
FBrf0246490-2.coIP.WB
Reference
Bajpai et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait

HA tag

vg 

vg

prey

FLAG tag

vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vg 
TONDU domain
sufficient binding region
aa 289-312

coordinates relative to vg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bajpai et al., 2020)
FBrf0246989-1.SPR
Reference
Mesrouze et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

bait
sd 
sd 
sd 
vg 

Vg

prey
vg 
vg 
vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
interaction domain
sufficient binding region
aa 223-440

coordinates relative to sd-PB

vg 
TONDU domain
sufficient binding region
aa 288-311

Kd = 291nM, coordinates relative to vg-PA

vg 
TONDU and Ω-loop domains
sufficient binding region
aa 288-337

Kd = 1.6nM, coordinates relative to vg-PA

vg 
α-helix of TONDU domain and Ω-loop domain
sufficient binding region
aa 298-337

Kd = 61nM, coordinates relative to vg-PA

sd 
interaction domain residue 276
mutation decreasing interaction
aa 276

D276A, coordinates relative to sd-PB

sd 
interaction domain residue 435
mutation decreasing interaction
aa 435

Y435H, coordinates relative to sd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Mesrouze et al., 2020)
FBrf0246989-3.XC
Reference
Mesrouze et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

Sd

neutral component
sd 

Sd

neutral component
sd 
sd 

Sd

neutral component
sd 
vg 

Vg

neutral component
vg 
vg 

Vg

neutral component
vg 

Vg

neutral component
vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
interaction domain
sufficient binding region
aa 223-440

coordinates relative to sd-PB

vg 
α-helix of TONDU domain and Ω-loop domain
sufficient binding region
aa 298-337

coordinates relative to vg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

(Mesrouze et al., 2020)
FBrf0250189-2.SPR
Reference
Mesrouze et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vg 

Vg

bait
sd 

Sd

prey
sd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
α-helix domain residues 288-311
sufficient binding region
aa 288-311

coordinates relative to vg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mesrouze et al., 2021)

positive control

(Mesrouze et al., 2021)
FBrf0252996-2.SPR
Reference
Mesrouze et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sd 

sd

bait

His-Avi tag

sd 
vg 

vg

prey

His tag

vg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sd 
YAP binding domain residue 350
mutation decreasing interaction
aa 350

K350A; within the context of aa 223-440.

vg 
interaction domain
sufficient binding region
aa 263-382
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Mesrouze et al., 2022)

0.38 +/- 0.02 nM for wild type sd compared with 2.3 +/- 0.3 for K350A sd.

(Mesrouze et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (11)