FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
msl-1, lncRNA:roX2
FlyBase ID
FBig0000097313
Interaction Network
Interactions Browser links
msl-1 network
lncRNA:roX2 network
Reported Interactions
FBrf0123205-11.coIP.PCR
Reference
Smith et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
msl-1 

MSL1

bait
lncRNA:roX2 

roX2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
(Smith et al., 2000)

Positive control.

(Smith et al., 2000)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Smith et al., 2000)
FBrf0125179-1.coIP.PCR
Reference
Meller et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
msl-1 

Msl1

bait
lncRNA:roX2 

roX2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Meller et al., 2000)
FBrf0220106-17.coIP.PCR
Reference
Hallacli et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-1 

Msl1

bait

FLAG tag

msl-1 
msl-1 
lncRNA:roX2 

roX2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
PEHE domain
mutation disrupting interaction
aa 886-979

E886R, F893R, H897R, F945E, A965E, F979E sextuple mutation

msl-1 
coiled coil
mutation disrupting interaction
aa 114-145aa 113-120

V114D, M121D, M142D, V145D quadruple mutation

M113R, L118R, L120R triple mutation

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Hallacli et al., 2012)

The interaction of msl-1 protein with roX2 RNA is disrupted by mutations that prevent the interaction of msl-1 with msl-2, msl-3 or mof

(Hallacli et al., 2012)
FBrf0246226-18.XL.WB
Reference
Müller et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lncRNA:roX2 

roX2

bait

biotin label

lncRNA:roX2 
msl-1 

MSL1

prey

Flag tag

msl-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lncRNA:roX2 
3 prime consensus box
sufficient binding region
msl-1 
delta N-terminal region residues 1-199
mutation decreasing interaction
aa 1-199

coordinates relative to msl-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Müller et al., 2020)
FBrf0261320-2.coIP.PCR
Reference
Babosha et al., 2024
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-1 

msl-1

bait

HA tag

msl-1 
msl-1 
lncRNA:roX2 

roX2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-1 
conserved N-terminal residues
necessary binding region
aa 3-7

K3S R4S F5G K6S W7G; coordinates relative to msl-1-PA

msl-1 
N-terminal region
necessary binding region
aa 41-85

coordinates relative to msl-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult flies; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Babosha et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)