fu and ci proteins coimmunoprecipitate from high molecular weight complexes fractionated by gel filtration.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Source was larval imaginal discs of fu mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

cos does not bind fu in the fu^RX2 mutant background.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

fu and ci interact only in the presence of Su(fu); a direct ci-fu interaction was not detected in a yeast two hybrid assay.

Three-hybrid system: yeast LexA-BD/B42-AD plus expression of Su(fu)

Source was imaginal discs of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

fu-ci interaction also occurs in a Su(fu) mutant background.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42A-AD

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction of ci and fu identified by anti-ci-phospho-S1382.