FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
sav, Sik3
FlyBase ID
FBig0000097550
Interaction Network
Interactions Browser links
sav network
Sik3 network
Reported Interactions
FBrf0220751-4.coIP.WB
Reference
Wehr et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Sik3 

Sik3

bait

FLAG tag

Sik3 
Sik3 
sav 

Sav

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sik3 
C-terminal region
necessary binding region
aa 693-1471

requires C-terminus present in \'PB\' but not \'PA\' isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wehr et al., 2013)
FBrf0220751-7.coIP.WB
Reference
Wehr et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

FLAG tag

Sik3 

Sik3

prey

HA tag

Sik3 
Sik3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sik3 
C-terminal region
necessary binding region
aa 693-1471

requires C-terminus present in \'PB\' but not \'PA\' isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wehr et al., 2013)

Cotransfection of Sik3 and sav stimulates the accumulation of Ser413-phosphorylated sav isoforms.

(Wehr et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)