Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from transfected S2 cell extract.

The mei-P26 3-prime UTR that binds bam also mediates translational repression in vivo.

The bgcn protein appears to bind RNA promiscuously, but it may be specifically targeted to the mei-P26 3-prime UTR as part of a bgcn-bam complex.

mei-P26, GFP-bgcn, Sxl and HA-bam cofractionate over a range of Superdex G-200 gel filtration fractions.

Source was adult female ovaries of transgenic fly line; proteins produced from endogenous gene or tagged transgenic construct.

mei-P26 coimmunoprecipitates bgcn in wild-type and bam mutant ovaries.

Source was adult female ovaries of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast lexA-BD/GAL4-AD