Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Mutations to N-terminal Glu residues in pan (E31K or E43K) reduces binding to arm by about five-fold.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Positive control.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

arm binds the pan-ci chimera encoded by the ci^D allele, but not to full length ci.

Binding was tested using the pan-ci chimera encoded by the ci^D allele.

The pan-ci chimera was immunoprecipitated using antibodies to full length ci.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

arm binds the pan-ci chimera encoded by the ci^D allele, but not to full length ci.

Binding was tested using the pan-ci chimera encoded by the ci^D allele.

Interaction in vitro; bait peptide synthesized in vitro; prey produced and labeled by in vitro translation.

Addition of 100-fold molar excess of lgs does not disrupt the pan-arm interaction.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from transfected construct.

The interaction of arm with pan is inhibited by oxazole, thiazole and thiazolidinedione.