Nuclear extract was purified through several different chromatographic steps, selecting for chromatin assembly and remodeling activity.
Identity of p140 as Iswi confirmed by western blot and mass spectrometry. Identity of p170 and p185 bands as Acf1 determined in subsequent publication (FBrf0109981). p47 determined to be a contaminant.
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.
Identity of CHRAC subunits determined in this and other publications by mass spectrometry (FBrf0129776, FBrf0138380).
Top2 later shown to be a contaminant that is not part of the CHRAC complex (FBrf0138380).
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Identity of p170 and p185 bands as Acf1 by peptide sequencing.
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Nuclear extract was purified through several different chromatographic steps, selecting for chromatin assembly and remodeling activity.
Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.
Identity of CHRAC subunits determined in this and other publications by mass spectrometry (FBrf0097684, FBrf0129776) and confirmed by western blot.
Top2 later shown to be a contaminant that is not part of the CHRAC complex (FBrf0138380).
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.
Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 cell line; prey produced as a recombinant fusion protein in bacterial system.
Source was cell extract of baculovirus-infected Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Positive control.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
