FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(bx), Iswi
FlyBase ID
FBig0000098405
Interaction Network
Interactions Browser links
E(bx) network
Iswi network
Reported Interactions
FBrf0086062-1.CH.MW
Reference
Tsukiyama et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

215 Kd

neutral component
E(bx) 

215 Kd

neutral component
E(bx) 

215 Kd

neutral component
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nuclear extract was purified through several different chromatographic steps, selecting for nucleosome disruption activity.

(Tsukiyama et al., 1995)

Identity of 140 kDa band as Iswi confirmed by both western blot and mass spectrometry.

(Tsukiyama et al., 1995)

E(bx), Iswi, Caf1 and Nurf-38 cosediment as part of the approximately 500 kDa NURF complex.

(Tsukiyama et al., 1995)

Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.

(Tsukiyama et al., 1995)
FBrf0086063-1.CH.MW
Reference
Tsukiyama and Wu, 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

215 Kd

neutral component
E(bx) 

215 Kd

neutral component
E(bx) 

215 Kd

neutral component
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Iswi 

140 Kd

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nuclear extract was purified through several different chromatographic steps, selecting for nucleosome disruption activity.

(Tsukiyama and Wu, 1995)

E(bx), Iswi, Caf1 and Nurf-38 cosediment as part of the approximately 500 kDa NURF complex.

(Tsukiyama and Wu, 1995)

Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.

(Tsukiyama and Wu, 1995)
FBrf0201622-2.coIP.WB
Reference
Ferreira et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

NURF301

bait
Iswi 

ISWI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ferreira et al., 2007)

Iswi that immunoprecipitated with E(bx) was acetylated on K753.

(Ferreira et al., 2007)
FBrf0201622-4.coIP.WB
Reference
Ferreira et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

NURF301

bait
Iswi 

ISWI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Iswi that immunoprecipitated with E(bx) was acetylated on K753.

(Ferreira et al., 2007)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ferreira et al., 2007)
FBrf0208433-5.coIP.WB
Reference
Kwon et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

Nurf301

bait

FLAG tag

E(bx) 
Iswi 

ISWI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(bx) 
interaction domain
sufficient binding region
aa 1-1557
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from recombinant baculovirus construct; prey produced from recombinant baculovirus construct.

(Kwon et al., 2009)
FBrf0230177-10.coIP.MS
Reference
Doyen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Iswi 

ISWI

bait
E(bx) 

NURF301

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doyen et al., 2015)
FBrf0230177-16.coIP.MS
Reference
Doyen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Iswi 

ISWI

bait
E(bx) 

NURF301

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Doyen et al., 2015)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doyen et al., 2015)
FBrf0230177-20.coIP.MS
Reference
Doyen et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

NURF301

bait
Iswi 

ISWI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Doyen et al., 2015)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Doyen et al., 2015)
FBrf0241907-25.coIP.WB
Reference
Lam et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
E(bx) 

NURF301

bait
Iswi 

ISWI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Lam et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)