FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sti, neb
FlyBase ID
FBig0000098450
Interaction Network
Interactions Browser links
sti network
neb network
Reported Interactions
FBrf0221857-1.coIP.MS
Reference
Bassi et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sti 

Sti

bait

protein A tag

sti 
neb 

Neb

prey

4 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sti 
coiled-coil CC1 domain
sufficient binding region
aa 463-783
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Bassi et al., 2013)
FBrf0221857-4.coIP.WB
Reference
Bassi et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sti 

Sti

bait

protein A tag

sti 
neb 

Neb

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sti 
coiled-coil CC1 domain
sufficient binding region
aa 463-783
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bassi et al., 2013)
FBrf0221857-6.PD.A
Reference
Bassi et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sti 

Sti

bait

GST tag

sti 
neb 

Neb

prey

[35]S label

neb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sti 
coiled-coil CC1 domain
sufficient binding region
aa 463-783
neb 
C-terminal region
sufficient binding region
aa 741-1121
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bassi et al., 2013)

sti binds directly to neb and pav but not feo.

(Bassi et al., 2013)

sti appears to form a ternary complex with neb and pav, as there is no apparent competition between neb and pav for binding to GST-sti.

(Bassi et al., 2013)
FBrf0221857-8.PD.A
Reference
Bassi et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
neb 

Neb

bait

GST tag

neb 
sti 

Sti

prey

[35]S label

sti 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
neb 
C-terminal region
sufficient binding region
aa 741-1121
sti 
coiled-coil CC1 domain
sufficient binding region
aa 463-783
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Bassi et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)