Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

brm, mor, dalao, Hsc70-4, Bap60, Bap55, Snr1 and Bap47 (actin) were identified as components of the Brahma complex.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before pull down of His tagged brm.

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated on a glycerol gradient and fractions containing brm and osa were used for immunoprecipitation.

brm, osa, Bap170, p400, mor, dalao, Bap60, Bap55, Snr1, Bap47 (actin) and Bap26 were identified as components of the Brahma complex.

Source was embryonic nuclear extract of wild-type fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Nuclear extract was fractionated through several different chromatographic steps, selecting for brm-containing fractions, before immunoprecipitation of brm.

brm, mor, dalao, Bap60, Bap55, Snr1 and actin form the Brahma (BRM) complex, together with either osa (BAP BRM complex) or polybromo and Bap170 (PBAP BRM complex).

Source was embryonic nuclear extract wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Nuclear extract was fractionated by ammonium sulfate precipitation and several chromatographic steps before being subjected to immunoprecipitation.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The brm-Act5C interaction is increased upon Tctp RNAi-knockdown.