FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, mir-13b-1
FlyBase ID
FBig0000098940
Interaction Network
Interactions Browser links
AGO1 network
mir-13b-1 network
Reported Interactions
FBrf0191204-1.coIP.NB
Reference
Rehwinkel et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

mir-13b-1 

miR-13b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mir-13b-1 (mature miRNA and pre-miRNA) coimmunoprecipitates with AGO1, and to a lesser extent, with AGO2 as well.

(Rehwinkel et al., 2006)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rehwinkel et al., 2006)
FBrf0204460-3.coIP.NB
Reference
Eulalio et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait

HA tag

AGO1 
mir-13b-1 

miR-13b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
central region
mutation decreasing interaction
aa 594-629

F594V, F629V \'F2V2\' mutant, coordinates relative to 984 residue isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eulalio et al., 2008)
FBrf0220359-5.coIP.NB
Reference
Pinder and Smibert, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

AGO1 
mir-13b-1 

miR-13b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AGO1 
MID domain
mutation disrupting interaction
aa 657-694

K657A, K694A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Pinder and Smibert, 2013)

Embryo extracts were crosslinked, immunoprecipitated and bound miRNA assayed by Northern blot.

(Pinder and Smibert, 2013)

Positive control.

(Pinder and Smibert, 2013)
External Crossreferences and Linkouts ( 0 )
References (3)