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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099062
Interaction Network
Interactions Browser links
Keap1 network
ref(2)P network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222430-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Keap1

bait

HA tag

p62

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

ref(2)P interacts with Keap1 but not Atg9.

The ref(2)P construct lacked the PB1 domain to prevent self-aggregation.

FBrf0225216-12.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Keap1

bait

HA tag

ref(2)P

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0228687-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DmKeap1

bait

FLAG tag

Ref(2)P

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
UBA domain
necessary binding region
aa 552-595

"ΔUBA" construct, coordinates relative to ref(2)P-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

A direct Keap1-ref(2)P interaction could not be detected in GST pull down assays.

FBrf0228687-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ref(2)P

bait

FLAG tag

DmKeap1

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
UBA domain
necessary binding region
aa 552-595

"ΔUBA" construct, coordinates relative to ref(2)P-PA

UBA domain residue M565
mutation disrupting interaction
aa 565

M565V, coordinates relative to ref(2)P-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

A direct Keap1-ref(2)P interaction could not be detected in GST pull down assays.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)