FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Keap1, ref(2)P
FlyBase ID
FBig0000099062
Interaction Network
Interactions Browser links
Keap1 network
ref(2)P network
Reported Interactions
FBrf0222430-1.coIP.WB
Reference
Nagy et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Keap1 

Keap1

bait

HA tag

ref(2)P 

p62

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Nagy et al., 2013)

ref(2)P interacts with Keap1 but not Atg9.

(Nagy et al., 2013)

The ref(2)P construct lacked the PB1 domain to prevent self-aggregation.

(Nagy et al., 2013)
FBrf0225216-12.coIP.WB
Reference
Hegedűs et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Keap1 

Keap1

bait

HA tag

ref(2)P 

ref(2)P

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hegedűs et al., 2014)
FBrf0228687-4.coIP.WB
Reference
Jain et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Keap1 

DmKeap1

bait

FLAG tag

ref(2)P 

Ref(2)P

prey

GFP tag

ref(2)P 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ref(2)P 
UBA domain
necessary binding region
aa 552-595

"ΔUBA" construct, coordinates relative to ref(2)P-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jain et al., 2015)

A direct Keap1-ref(2)P interaction could not be detected in GST pull down assays.

(Jain et al., 2015)
FBrf0228687-5.coIP.WB
Reference
Jain et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
ref(2)P 

Ref(2)P

bait

FLAG tag

ref(2)P 
ref(2)P 
Keap1 

DmKeap1

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ref(2)P 
UBA domain
necessary binding region
aa 552-595

"ΔUBA" construct, coordinates relative to ref(2)P-PA

ref(2)P 
UBA domain residue M565
mutation disrupting interaction
aa 565

M565V, coordinates relative to ref(2)P-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jain et al., 2015)

A direct Keap1-ref(2)P interaction could not be detected in GST pull down assays.

(Jain et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)