Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Positive control.
Cells were treated with the proteasome inhibitor MG132 to increase the mitotic index of the cell culture and enrich for mitotic complexes.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was cell extract of S2 cell line; proteins produced from endogenous genes.
