Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

From a panel of 27 small G proteins tested, cbs interacted with Arl1, Rab6, Rab19 and Rab30.

cbs binds to the activated (GTP-locked) forms of small G proteins (Q-to-L mutation).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

The GTP-bound form of Rab6 binds CG10703 and cbs.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell detergent-free extract (endogenous gene).

Specificity S-score=19.18.

Constitutively active (GTP-locked) form of Rab was used for pull down. Parallel pull downs performed for several Rabs proteins in this study. Yield was measured using spectral counts. A specificity score (S score) was calculated, where greater weight was given to interactions seen with fewer baits.