FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mer
FlyBase ID
FBig0000099296
Interaction Network
Interactions Browser links
Mer network
Reported Interactions
FBrf0214876-1.PD.WB
Reference
Benseñor et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mer 

Merlin

bait

GFP tag

Mer 

Merlin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Benseñor et al., 2010)
FBrf0222652-3.coIP.WB
Reference
Yin et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Mer 

Mer

bait

FLAG tag

Mer 

Mer

prey

HA tag

Mer 
Mer 
Mer 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
N-terminal region
sufficient binding region
aa 1-375
Mer 
C-terminal region
sufficient binding region
aa 375-635
Mer 
C-terminal region
necessary binding region
aa 601-635
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yin et al., 2013)

The C-terminal region of Mer interacts with the N-terminal region of Mer. This intramolecular interaction presumably regulates the activity of Mer, and explains why C-terminal truncations of Mer exhibit gain-of-function activity.

(Yin et al., 2013)
FBrf0248717-1.ITC
Reference
Zhang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Merlin

bait

sumo tag, FERM domain

Mer 

Merlin

prey

Trx tag, CTD domain

Mer 
Mer 
Mer 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 9-313
Mer 
CTD domain
sufficient binding region
aa 516-635
Mer 
CTD domain residue 616
mutation decreasing interaction
aa 616

T616D, Kd = 14uM

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2021)

Kd = 20nM

(Zhang et al., 2021)
FBrf0248717-2.CH.MW
Reference
Zhang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Merlin

Merlin

neutral component

FERM domain

CTD domain

Mer 
Mer 
Mer 
Mer 

Merlin

Merlin

neutral component

FERM domain

CTD domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 9-313
Mer 
CTD domain
sufficient binding region
aa 516-635
Mer 
CTD domain residue 616
mutation decreasing interaction
aa 616

T616D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2021)
FBrf0248717-3.CS.MW
Reference
Zhang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Merlin

Merlin

neutral component

FERM domain

CTD domain

Mer 
Mer 
Mer 

Merlin

Merlin

neutral component

FERM domain

CTD domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 9-313
Mer 
CTD domain
sufficient binding region
aa 516-635
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Mer FERM/CTD complex is a 1:1 heterodimer.

(Zhang et al., 2021)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2021)
FBrf0248717-4.XC
Reference
Zhang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mer 

Merlin

Merlin

neutral component

FERM domain

CTD domain

Mer 

Merlin

Merlin

neutral component

FERM domain

CTD domain

Mer 
Mer 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mer 
FERM domain
sufficient binding region
aa 9-313
Mer 
CTD domain
sufficient binding region
aa 516-635
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Zhang et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (3)