FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdc42, par-6
FlyBase ID
FBig0000099487
Interaction Network
Interactions Browser links
Cdc42 network
par-6 network
Reported Interactions
FBrf0174952-2.PD
Reference
Peterson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

par-6 

Par-6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system.

(Peterson et al., 2004)
FBrf0174952-3.NMR
Reference
Peterson et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

neutral component
Cdc42 

Cdc42

neutral component
Cdc42 

Cdc42

neutral component
par-6 

Par-6

neutral component
par-6 
par-6 

Par-6

neutral component
par-6 

Par-6

neutral component
par-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
CRIB-PDZ
sufficient binding region
aa 130-255

coordinates relative to par-6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Peterson et al., 2004)
FBrf0179253-2.PD.A
Reference
Hutterer et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

par-6 
Cdc42 

Cdc42

prey

[35]S label

Cdc42 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cdc42 
region
mutation disrupting interaction
aa 17

T17N

par-6 
CRIB domain
mutation disrupting interaction
aa 139

\'ΔP\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Hutterer et al., 2004)

par-6 interacts with Cdc42 but not Rac1.

(Hutterer et al., 2004)

GDP-bound, dominant negative Cdc42[N17] does not bind to par-6.

(Hutterer et al., 2004)
FBrf0200720-1.PD.A
Reference
Atwood et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

par-6 

Par-6

prey
par-6 
par-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
semi-CRIB domain
mutation disrupting interaction
aa 136-137

Double alanine substitution.

par-6 
semi-CRIB domain
mutation decreasing interaction
aa 139aa 142

P139A

F142A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Atwood et al., 2007)

Cdc42 is loaded with GTP in this experiment.

(Atwood et al., 2007)
FBrf0243203-1.PD.WB
Reference
Nunes de Almeida et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Cdc42 

Cdc42

bait

GST tag

Cdc42 
par-6 

Par6

prey

FLAG tag

par-6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
CRIB domain
mutation disrupting interaction
aa 139

deletion of P139

Cdc42 
N-terminal domain residue 17
mutation disrupting interaction
aa 17

T17N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Cdc42-par-6 interaction was shown using the V12 constitutively active form of Cdc42.

(Nunes de Almeida et al., 2019)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cell extract.

(Nunes de Almeida et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)