FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Leash, yki
FlyBase ID
FBig0000100092
Interaction Network
Interactions Browser links
Leash network
yki network
Reported Interactions
FBrf0223213-20.coIP.WB
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Leash 

leash

bait

HA tag

Leash 
yki 

yki

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Leash 
ubiquitinated lysine residues
mutation decreasing interaction
aa 74-148

K74R, K116R and K148R: \'3K/R\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kwon et al., 2013)
FBrf0223213-24.coIP.WB
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Myc tag

Leash 

Leash

prey

HA tag

Leash 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Leash 
Arrestin domains
sufficient binding region
aa 1-305
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kwon et al., 2013)
FBrf0224345-189.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

Leash 

CG4674

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0224619-25.coIP.WB
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Leash 

Leash

bait

HA tag

yki 

yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)

Top 50 prey proteins from Leash AP/MS are reported in supplementary table S8. Only the validated yki-Leash interaction was curated for FlyBase.

(Kwon et al., 2013)
FBrf0238240-1.coIP.WB
Reference
Shen et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

Leash 

Leash

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shen et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)