FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
yki, mop
FlyBase ID
FBig0000100157
Interaction Network
Interactions Browser links
yki network
mop network
Reported Interactions
FBrf0213686-1.TAP.MS
Reference
Gilbert et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mop 

mop

bait

Protein G and SBP tag

yki 

yki

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey derived from endogenous gene.

(Gilbert et al., 2011)

mop mutation C1728A used in purification

(Gilbert et al., 2011)
FBrf0213686-2.coIP.WB
Reference
Gilbert et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

HA Tag

yki 
yki 
mop 

mop

prey

V5 Tag

mop 
mop 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domain residue 281
mutation disrupting interaction
aa 281

Y281A, coordinates relative to yki-PF

yki 
WW domain residue 350
mutation disrupting interaction
aa 350

Y350A, coordinates relative to yki-PF

mop 
interaction domain residue 791
mutation disrupting interaction
aa 791

Y791A

mop 
interaction domain residue 823
mutation disrupting interaction
aa 823

Y823A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gilbert et al., 2011)

Both wild type mop and mutation C1728A used in co-IP with yki with no difference in binding.

(Gilbert et al., 2011)
FBrf0224345-197.coIP.MS
Reference
Kwon et al., 2013
Description
physical association
Assay
Collection
Hippo_Pathway_Interactome
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
yki 

yki

bait

Protein A tag, Strep tag

mop 

mop

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2013)
FBrf0232944-3.PD.MS
Reference
Hu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

GST tag

yki 
mop 

Mop

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
WW domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Hu et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)