Source was live embryos of wild-type fly line; bait produced from endogenous gene; prey derived from baculovirus-infected insect cells.

Binding of Fc-tagged drl to the posterior commissure of live embryos is abolished in Wnt5 mutant embryos. Ubiquitous expression of Wnt5 (but not Wnt4 or wg) results in an expansion of Fc-drl binding to all axon tracts.

Live embryos were dissected and incubated with Fc-tagged drl for 30 minutes at room temperature before fixation.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected insect cell system; prey derived from wild-type embryonic extract.

Fc-tagged drl pulls down both the 140kDa and 80kDa forms of Wnt5 from wild-type embryo extracts, but not from Wnt5 mutant embryo extracts. No pull down of wg was observed.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey derived from transfected S2 cell extract.

S2 cells were transfected with Wnt5 and Drl-2, then after 48 hours, cells were washed and resuspended in serum-free medium in the presence of Fc-tagged drl.