FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000100693
Interaction Network
Interactions Browser links
Wnt5 network
drl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0157164-1.AT.TV
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnt5 

Wnt5

bait
drl 

Drl

prey

Fc tag

drl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live embryos of wild-type fly line; bait produced from endogenous gene; prey derived from baculovirus-infected insect cells.

Binding of Fc-tagged drl to the posterior commissure of live embryos is abolished in Wnt5 mutant embryos. Ubiquitous expression of Wnt5 (but not Wnt4 or wg) results in an expansion of Fc-drl binding to all axon tracts.

Live embryos were dissected and incubated with Fc-tagged drl for 30 minutes at room temperature before fixation.

FBrf0157164-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
drl 

Drl

bait

Fc tag

drl 
Wnt5 

Wnt5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected insect cell system; prey derived from wild-type embryonic extract.

Fc-tagged drl pulls down both the 140kDa and 80kDa forms of Wnt5 from wild-type embryo extracts, but not from Wnt5 mutant embryo extracts. No pull down of wg was observed.

FBrf0228474-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
drl 

DRL

bait

Fc tag

drl 
Wnt5 

WNT5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey derived from transfected S2 cell extract.

S2 cells were transfected with Wnt5 and Drl-2, then after 48 hours, cells were washed and resuspended in serum-free medium in the presence of Fc-tagged drl.

FBrf0251592-10.HDX.MS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnt5 

DWnt-5

neutral component
Wnt5 

DWnt-5

neutral component
Wnt5 

DWnt-5

neutral component
drl 

Drl

neutral component

His tag

drl 

Drl

neutral component

His tag

drl 

Drl

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.

FBrf0251592-2.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnt5 

DWnt-5

bait
drl 

Drl

prey

His tag

drl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
WIF domain residues 1-183
sufficient binding region
aa 1-183
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.

FBrf0251592-3.SPR
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wnt5 

DWnt-5

bait
Wnt5 
drl 

Drl

prey

His tag

drl 
drl 
drl 
drl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
WIF domain residues 1-183
sufficient binding region
aa 1-183
drl 
WIF domain residue 52
mutation disrupting interaction
aa 52

Y52E

drl 
WIF domain residue 58
mutation disrupting interaction
aa 58

V58E

drl 
WIF domain residue 56
mutation disrupting interaction
aa 56

F56E

Wnt5 
interaction domain residues 455-1004
sufficient binding region
aa 455-1004
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.

FBrf0251592-6.SPR
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
drl 

Drl

bait

His tag

drl 
Wnt5 

DWnt-5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
ECR domain residues 1-242
sufficient binding region
aa 1-242
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in Sf9 cells; prey produced from a transfected construct.

FBrf0251592-9.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
drl 

Drl

bait

Flag tag

drl 
Wnt5 

DWnt-5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
drl 
ECR domain residues 1-242
sufficient binding region
aa 1-183
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)