Source was live embryos of wild-type fly line; bait produced from endogenous gene; prey derived from baculovirus-infected insect cells.
Live embryos were dissected and incubated with Fc-tagged drl for 30 minutes at room temperature before fixation.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected insect cell system; prey derived from wild-type embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey derived from transfected S2 cell extract.
Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.
Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.
Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.
Interaction in vitro; bait produced as a recombinant fusion protein in Sf9 cells; prey produced from a transfected construct.
Interaction in vitro; bait produced from a transfected construct; prey produced as a recombinant fusion protein in Sf9 cells.