FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Toll-7, spz5
FlyBase ID
FBig0000100985
Interaction Network
Interactions Browser links
Toll-7 network
spz5 network
Reported Interactions
FBrf0222474-5.CS.WB
Reference
McIlroy et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spz5 

DNT2

neutral component

His tag

spz5 

DNT2

neutral component

His tag

spz5 
spz5 

DNT2

neutral component

His tag

spz5 
Toll-7 

Toll-7

neutral component

His tag

Toll-7 
Toll-7 

Toll-7

neutral component

His tag

Toll-7 

Toll-7

neutral component

His tag

Toll-7 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spz5 
cystine knot domain
sufficient binding region
aa 285-387
Toll-7 
extracellular domain
sufficient binding region
aa 1-1047
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus-infected Sf9 cell system.

(McIlroy et al., 2013)
FBrf0235455-7.coIP.WB
Reference
Foldi et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spz5 

spz5

bait

V5 tag

Toll-7 

Toll-7

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Foldi et al., 2017)
FBrf0242805-12.coIP.WB
Reference
Chowdhury et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ATCC
Participants
Corresponds to
Reported as
Role
Note
spz5 

Spz-5

bait

FLAG tag

Toll-7 

Toll-7

prey

V5 tag

Toll-7 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Toll-7 
ecto domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chowdhury et al., 2019)
FBrf0242805-6.coIP.WB
Reference
Chowdhury et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ATCC
Participants
Corresponds to
Reported as
Role
Note
Toll-7 

Toll-7

bait

V5 tag

Toll-7 
spz5 

Spz-5

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Toll-7 
ecto domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chowdhury et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)