Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene..
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
TAP tandem affinity purification of bait and identification of prey by mass spectrometry.
Source was nuclear extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Positive control.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 nuclear extract.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was adult flies of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
coordinates relative to CTCF-PA
coordinates relative to CTCF-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system
coordinates relative to CTCF-PA
coordinates relative to CTCF-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system
coordinates relative to CTCF-PA
coordinates relative to CTCF-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system
coordinates relative to CTCF-PA
coordinates relative to CTCF-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system
coordinates relative to CTCF-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
coordinates relative to Cp190-PA.
coordinates relative to Cp190-PA.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was from cDNA library of 154 Drosophila C2H2 domain proteins).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
within the context of aa 1-293; coordinates relative to Cp190-PA
Two-hybrid system: yeast GAL4-BD/VP16-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Positive control.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
