FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cap-H2, MRG15
FlyBase ID
FBig0000101221
Interaction Network
Interactions Browser links
Cap-H2 network
MRG15 network
Reported Interactions
FBrf0222628-1.Y2H
Reference
Smith et al., 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cap-H2 

Cap-H2

bait

fused to GAL4 DNA-binding domain

MRG15 

Mrg15

prey

fused to GAL4 activation domain

MRG15 
MRG15 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
MRG15 
C-terminal region
sufficient binding region
aa 256-424
MRG15 
C-terminal region
necessary binding region
aa 256-305aa 395-424
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

(Smith et al., 2013)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Smith et al., 2013)

Of 84 positive clones, three represented MRG15 cDNAs.

(Smith et al., 2013)
FBrf0222628-2.coIP.WB
Reference
Smith et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cap-H2 

Cap-H2

bait

GFP tag

MRG15 

Mrg15

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Smith et al., 2013)
FBrf0222628-3.coIP.WB
Reference
Smith et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
MRG15 

Mrg15

bait

V5 tag

Cap-H2 

Cap-H2

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Smith et al., 2013)
FBrf0222628-4.coIP.WB
Reference
Smith et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cap-H2 

Cap-H2

bait

GFP tag

MRG15 

Mrg15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Smith et al., 2013)
FBrf0228324-1.coIP.WB
Reference
Wallace et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cap-H2 

Cap-H2

bait

GFP tag

Cap-H2 
MRG15 

MRG15

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cap-H2 
interaction domain
mutation disrupting interaction
aa 510,512,513

F510A,L512A,P513A, coordinates relative to Cap-H2-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wallace et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)