FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dock, Pak
FlyBase ID
FBig0000101340
Interaction Network
Interactions Browser links
dock network
Pak network
Reported Interactions
FBrf0108798-1.Y2H
Reference
Hing et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

fused to LexA DNA-binding domain

dock 
dock 
Pak 

Pak

prey

fused to GAL4 activation domain

Pak 
Pak 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dock 
SH3-2 domain
sufficient binding region
dock 
SH3-2 domain
mutation disrupting interaction
aa 151

W151K, coordinates relative to 410-residue dock polypeptide

Pak 
N-terminal region
sufficient binding region

lacks kinase domain

Pak 
PXXP site
mutation disrupting interaction
aa 9aa 13

P9L

R13M

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hing et al., 1999)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Hing et al., 1999)
FBrf0108798-2.coIP.WB
Reference
Hing et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait
Pak 

Pak

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hing et al., 1999)
FBrf0167957-5.Y2H
Reference
Fan et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak 

Pak

bait

fused to LexA DNA-binding domain

dock 

Dock

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Fan et al., 2003)

dock interacts with Pak, but not Rac1, Rac2 or Mtl in this assay.

(Fan et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fan et al., 2003)
FBrf0214807-3.Y2H
Reference
Huang et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dock 

Dock

bait

fused to GAL4 DNA-binding domain

Pak 

Pak

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Huang et al., 2011)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Huang et al., 2011)
FBrf0214807-4.Y2H
Reference
Huang et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pak 

Pak

bait

fused to GAL4 DNA-binding domain

dock 

Dock

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Huang et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Huang et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)