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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000101803
Interaction Network
Interactions Browser links
rpr network
Diap1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0149132-3.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rpr 

Rpr

bait

GST tag

rpr 

DIAP1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rpr 
RHG motif
necessary binding region
aa 1-8
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Positive control.

FBrf0174987-10.XC
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rpr 

Reaper

neutral component
rpr 
rpr 

Reaper

neutral component
rpr 
rpr 

Reaper

neutral component

DIAP1

neutral component

DIAP1

neutral component

DIAP1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BIR1 domain
sufficient binding region
aa 30-135

coordinates relative to Diap1-PA

rpr 
N-terminal domain
sufficient binding region
aa 2-11

coordinates relative to rpr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Diap1 produced as a recombinant fusion proteins in bacterial system; rpr peptide chemically synthesized.

FBrf0174987-5.CH.MW
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rpr 

Reaper

neutral component
rpr 
rpr 

Reaper

neutral component
rpr 
rpr 

Reaper

neutral component

DIAP1

neutral component

DIAP1

neutral component

DIAP1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BIR1 domain
sufficient binding region
aa 30-145

coordinates relative to Diap1-PA

rpr 
N-terminal domain
sufficient binding region
aa 2-11

coordinates relative to rpr-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; Diap1 produced as a recombinant fusion proteins in bacterial system; rpr peptide chemically synthesized.

FBrf0174987-8.ITC
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait
rpr 

Reaper

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Kd = 0.27uM

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.

FBrf0184187-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

GST tag

rpr 

Rpr

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal region
sufficient binding region
aa 1-146

coordinates relative to Diap1-PA

N-terminal region residue C80
mutation disrupting interaction
aa 80

C80G, coordinates relative to Diap1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected cell extract.

FBrf0201142-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

GST tag

rpr 

Rpr

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0206305-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rpr 

Reaper

bait

GTC tag

DIAP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from transfected S2 cells; prey derived from transfected 293 cell lysate.

FBrf0211820-10.coIP.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Diap1

bait

FLAG tag

rpr 

rpr

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)