FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
AGO1, nanos
FlyBase ID
FBig0000101971
Interaction Network
Interactions Browser links
AGO1 network
nanos network
Reported Interactions
FBrf0220359-3.coIP.PCR
Reference
Pinder and Smibert, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait
nanos 

nanos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Pinder and Smibert, 2013)

The interaction of AGO1 with nos mRNA is not disrupted by mutations in AGO1 (K657A, K694A) that disrupt its binding to miRNAs.

(Pinder and Smibert, 2013)

AGO1 is recruited to nos mRNA in a miRNA-independent, smg-dependent process.

(Pinder and Smibert, 2013)

smg and AGO1, but not AGO2, repress the translation of unlocalized nos RNA in early embryos.

(Pinder and Smibert, 2013)

Embryo extracts were crosslinked, immunoprecipitated and the level of specific coimmunoprecipitated mRNAs was quantified by RT-qPCR.

(Pinder and Smibert, 2013)
FBrf0247403-13.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

nanos 

Nos

prey
nanos 
nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR proximal
sufficient binding region
nt 21-100
nanos 
3-prime UTR miRNA binding sites
mutation disrupting interaction
nt 34-40,76-81

GGCAGCU to GGUAGUU and CAUUCC to CAUUUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2020)
FBrf0247403-6.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

Ago1

bait

FLAG tag

nanos 

Nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Malik et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)