FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
flfl, Cenp-C
FlyBase ID
FBig0000102145
Interaction Network
Interactions Browser links
flfl network
Cenp-C network
Reported Interactions
FBrf0227269-1.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
flfl 

Falafel

bait

Protein A tag

flfl 
flfl 
Cenp-C 

CENP-C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
N-terminal region
sufficient binding region
aa 1-168
flfl 
N-terminal region
necessary binding region
aa 1-168
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-14.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Falafel

bait

Protein A tag

Cenp-C 

CENP-C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-18.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

FLAG tag

Cenp-C 
flfl 

Falafel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
Falafel interacting motif (FIM)
necessary binding region
aa 1048-1066
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-23.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

Protein A tag

flfl 

Falafel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-30.PD.A
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

GST tag

Cenp-C 
Cenp-C 
flfl 

Falafel

prey

[35]S label

flfl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
N-terminal region
sufficient binding region
aa 1-168
Cenp-C 
Falafel binding domain (FBD)
sufficient binding region
aa 1002-1093
Cenp-C 
Falafel interacting motif (FIM)
necessary binding region
PDESSADVVFKKPLAPAPR
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lipinszki et al., 2015)

Cenp-C binds to flfl but not with other components of the PP4 complex, Pp4-19C and PPP4R2r.

(Lipinszki et al., 2015)
FBrf0227269-31.PD.A
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Falafel

bait

GST tag

flfl 
flfl 
Cenp-C 

CENP-C

prey

[35]S label

Cenp-C 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
N-terminal region
sufficient binding region
aa 1-168
flfl 
N-terminal region
necessary binding region
aa 1-168
Cenp-C 
Falafel binding domain (FBD)
sufficient binding region
aa 1002-1093
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lipinszki et al., 2015)
FBrf0227269-32.coIP.WB
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

FLAG tag

Cenp-C 
flfl 

Falafel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
Falafel binding domain (FBD)
sufficient binding region
aa 1002-1093
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
FBrf0227269-37.PD.WB
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

GST tag

Cenp-C 
flfl 

Falafel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
Falafel binding domain (FBD)
sufficient binding region
aa 1002-1093
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Lipinszki et al., 2015)
FBrf0227269-38.CH.MW
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

neutral component
Cenp-C 

CENP-C

neutral component
Cenp-C 
Cenp-C 

CENP-C

neutral component
Cenp-C 
flfl 

Falafel

neutral component
flfl 
flfl 

Falafel

neutral component
flfl 

Falafel

neutral component
flfl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
Falafel binding domain (FBD)
sufficient binding region
aa 1002-1093
flfl 
N-terminal region
sufficient binding region
aa 1-168
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lipinszki et al., 2015)

Purified Cenp-C and flfl form a stable complex in vitro, as judged by size exclusion chromatography.

(Lipinszki et al., 2015)
FBrf0227269-39.AT.WB
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait
Cenp-C 
flfl 

Falafel

prey
flfl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
Falafel interacting motif (FIM)
sufficient binding region
PDESSADVVFKKPLAPAPR
flfl 
N-terminal region
sufficient binding region
aa 1-168
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lipinszki et al., 2015)

Purified Cenp-C and flfl form a stable complex in vitro, as judged by size exclusion chromatography.

(Lipinszki et al., 2015)

82 20-mer peptides, each shifted by 1 aa and covering the whole Cenp-C FBD (aa 1002-1093), were spotted onto cellulose membrane. High purity flfl was added, washed off, and binding detected anti-flfl western blot.

(Lipinszki et al., 2015)
FBrf0227269-40.AT.WB
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait
Cenp-C 
Cenp-C 
flfl 

Falafel

prey
flfl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cenp-C 
Falafel interacting motif (FIM)
sufficient binding region
PDESSADVVFKKPLAPAPR
Cenp-C 
Falafel interacting motif (FIM)
necessary binding region
FKKP
flfl 
N-terminal region
sufficient binding region
aa 1-168
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lipinszki et al., 2015)

Purified Cenp-C and flfl form a stable complex in vitro, as judged by size exclusion chromatography.

(Lipinszki et al., 2015)

380 variants of the 19-mer Falafel interacting motif (FIM) were generated, substituting each residue with all possible amino acids. Peptides were spotted onto nitrocellulose, incubated with flfl, and binding detected by anti-flfl western blot.

(Lipinszki et al., 2015)
FBrf0227269-41.coIP.WB
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
flfl 

Flfl

bait

GFP tag

flfl 
flfl 
Cenp-C 

CENP-C

prey

FLAG tag

Cenp-C 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
N-terminal region
sufficient binding region
aa 1-168
flfl 
N-terminal region
necessary binding region
aa 1-168
Cenp-C 
Falafel interacting motif (FIM)
necessary binding region
PDESSADVVFKKPLAPAPR
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lipinszki et al., 2015)
FBrf0227269-42.XC
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
flfl 

Flfl

neutral component
flfl 

Flfl

neutral component
flfl 
flfl 

Flfl

neutral component
flfl 
Cenp-C 

CENP-C

neutral component
Cenp-C 
Cenp-C 

CENP-C

neutral component
Cenp-C 

CENP-C

neutral component
Cenp-C 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
flfl 
EVH1 domain
sufficient binding region
aa 1-122
Cenp-C 
Falafel interacting motif (FIM)
sufficient binding region
PDESSADVVFKKPLAPAPR
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; peptide chemically synthesized in vitro.

(Lipinszki et al., 2015)
FBrf0227269-5.coIP.MS
Reference
Lipinszki et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Cenp-C 

CENP-C

bait

Protein A tag

flfl 

Falafel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lipinszki et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)