Source was whole cell extracts; bait produced from endogenous gene; prey produced from endogenous gene.
treatment with histone deacetylase inhibitor trichostatin A (TSA) reduced roX2 interaction
acetylation of msl-3 disrupts interaction with lncRNA:roX2 ; acetylated mutant msl-3 K110-112G disrupts interaction with lncRNA:roX2 .
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in a baculovirus system
MSL3 binds to RNA significantly better than dsDNA
Positive control.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Bound RNA was sequenced.
Positive control.
