acetylation of msl-3 disrupts interaction with lncRNA:roX2 ; acetylated mutant msl-3 K110-112G disrupts interaction with lncRNA:roX2 .
Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Source was cross-linked nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Crude nuclear extracts were cross-linked using formaldehyde, sonicated, and subjected to ProteinA affinity purification with rabbit IgG agarose beads eluted under denaturing conditions, and subsequently using streptavidin agarose beads for Bio affinity purification. Bound RNA was sequenced.