FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ALiX, shrb
FlyBase ID
FBig0000102519
Interaction Network
Interactions Browser links
ALiX network
shrb network
Reported Interactions
FBrf0227451-1.coIP.WB
Reference
Eikenes et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
shrb 

Shrub

bait

GFP tag

ALiX 

ALIX

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eikenes et al., 2015)
FBrf0227451-2.coIP.WB
Reference
Eikenes et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ALiX 

ALIX

bait

GFP tag

ALiX 
shrb 

Shrub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ALiX 
interaction domain
mutation disrupting interaction
aa 198aa 211

F198D

I211D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Eikenes et al., 2015)

The interaction between human orthologs of ALiX and shrb have been previously shown to depend on residues corresponding to residues F198 and I211 in ALiX.

(Eikenes et al., 2015)
FBrf0243783-5.PD.A
Reference
Lie-Jensen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ALiX 

ALIX

bait

GST tag

shrb 

Shrub

prey

Myc tag, [35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lie-Jensen et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)