tut, bam Physical Interaction report

Collection

Source/Stage

testis / adult stage male

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tut

Tut

bait

TAP tag

bam

Bam

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult male testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chen et al., 2014)

FBrf0226802-16.coIP.WB

Reference

Chen et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tut

Tut

bait

HA tag

bam

Bam

prey

FLAG tag

bam

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

bam

N-terminal region

sufficient binding region

aa 1-100

bam

N-terminal region

necessary binding region

aa 1-100

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)

FBrf0226802-17.coIP.WB

Reference

Chen et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bam

Bam

bait

HA tag

bam

tut

Tut

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

bam

N-terminal region

sufficient binding region

aa 1-100

bam

N-terminal region

necessary binding region

aa 1-100

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)

FBrf0226802-3.Y2H

Reference

Chen et al., 2014

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bam

Bam

bait

fused to DNA-binding domain

bam

tut

Tut

prey

fused to activation domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

bam

N-terminal region

sufficient binding region

aa 1-100

bam

N-terminal region

necessary binding region

aa 1-100

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast

(Chen et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen et al., 2014)

Pairwise yeast two-hybrid interactions between tut-bam and bgcn-bam, but not tut-bgcn.

(Chen et al., 2014)

FBrf0226802-4.Y2H

Reference

Chen et al., 2014

Description

physical association

Assay

two hybrid

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tut

Tut

bait

fused to activation domain

bam

Bam

prey

fused to DNA-binding domain

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Two-hybrid system: yeast

(Chen et al., 2014)

Pairwise yeast two-hybrid interactions between tut-bam and bgcn-bam, but not tut-bgcn.

(Chen et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Chen et al., 2014)

FBrf0226802-5.coIP.WB

Reference

Chen et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tut

Tut

bait

Myc tag

bam

Bam

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)

FBrf0226802-6.coIP.WB

Reference

Chen et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bam

Bam

bait

FLAG tag

tut

Tut

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)

FBrf0226802-8.coIP.WB

Reference

Chen et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

tut

Tut

bait

TAP tag

bam

Bam

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Cells were transfected with tagged tut, bgcn and bam.

(Chen et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2014)

FBrf0234954-13.coIP.WB

Reference

Shan et al., 2017

Description

physical association

Assay