Ubr3, Diap1 Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

GTC tag

Diap1

Ubr3

UBR3

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap1

interaction domain

sufficient binding region

aa 21-438

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ditzel et al., 2008)

FBrf0226660-1.coIP.WB

Reference

Huang et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Ubr3

bait

FLAG tag

Ubr3

Diap1

DIAP1

prey

V5 tag

Diap1

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap1

ΔN form

sufficient binding region

aa 21-438

mimics the caspase cleaved form

Diap1

neoepitope

mutation disrupting interaction

aa 21

N21M

Ubr3

N-terminal and central regions

sufficient binding region

aa 1-1514

ΔRING construct, coordinates relative to Ubr3-PA

Ubr3

UBR domain

necessary binding region

aa 222-292

ΔUBR construct, coordinates relative to Ubr3-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2014)

Ubr3 binds more strongly to N-terminally cleaved Diap1.

(Huang et al., 2014)

FBrf0226660-2.coIP.WB

Reference

Huang et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

V5 tag

Diap1

Ubr3

prey

FLAG tag

Ubr3

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap1

ΔN form

sufficient binding region

aa 21-438

mimics the caspase cleaved form

Diap1

neoepitope

mutation disrupting interaction

aa 21

N21M

Ubr3

N-terminal and central regions

sufficient binding region

aa 1-1514

ΔRING construct, coordinates relative to Ubr3-PA

Ubr3

UBR domain

necessary binding region

aa 222-292

ΔUBR construct, coordinates relative to Ubr3-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Ubr3 binds more strongly to N-terminally cleaved Diap1.

(Huang et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2014)

FBrf0226660-7.coIP.WB

Reference

Huang et al., 2014

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

V5 tag

Ubr3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2014)

FBrf0229651-10.coIP.WB

Reference

Zanet et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Ubr3

bait

GFP tag

Diap1

DIAP1

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zanet et al., 2015)

Cells treated with MG132 to stabilize proteins from proteasomal degradation.

(Zanet et al., 2015)

FBrf0229651-9.coIP.WB

Reference

Zanet et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

FLAG tag

Ubr3

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zanet et al., 2015)

Cells treated with MG132 to stabilize proteins from proteasomal degradation.

(Zanet et al., 2015)

FBrf0258079-1.coIP.WB

Reference

Markus et al., 2023

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

GFP tag

Ubr3

UBR3

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Addition of tal peptides increase Diap1-Ubr3 interaction.

(Markus et al., 2023)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Markus et al., 2023)

FBrf0258079-2.coIP.WB

Reference

Markus et al., 2023

Description

physical association

Assay

Collection

Source/Stage

Cell line used