FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103179
Interaction Network
Interactions Browser links
Nrg network
Reported Interactions
FBrf0092483-2.BA.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrg 

Nrg

self
Nrg 

Nrg

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

Nrg can mediate homophilic adhesion.

Source was live S2 cells; proteins produced from transfected constructs.

FBrf0195131-2.BA.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrg 

Nrg

self
Nrg 

Nrg

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

S2 cells were transfected with GFP and Nrg expression vectors and aggregation was observed with transmission and confocal microscopy.

FBrf0213316-1.BA.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrg 

NRG

bait
Nrg 

NRG

prey
Nrg 
Nrg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrg 
Ig2 domain surface
mutation disrupting interaction
aa 213

S213L, coordinates relative to 180 kD isoform repoted in FBrf0103453.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

FBrf0242199-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrg 

Nrg

bait

180kD isoform

Nrg 

Nrg

prey

GFP tag, 167 kD isoform

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

FBrf0242199-2.BA.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrg 

Nrg

Nrg

neutral component

mCherry tag, 180kD isoform

GFP tag, 167 kD isoform

Nrg 

Nrg

Nrg

neutral component

mCherry tag, 180kD isoform

GFP tag, 167 kD isoform

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

External Crossreferences and Linkouts ( 0 )
References (4)