Interaction in vitro; proteins produced by in vitro translation.
Though each dpn and E(spl)mγ-HLH can bind to DNA independently, novel DNA-protein complexes are formed when dpn and E(spl)mγ-HLH are mixed together, suggesting the formation of heterodimers.
Proteins were incubated with a DNA probe containing E[[B]]-box motifs.
The presence of insb disrupts dpn-E(spl)mγ-HLH dimerization.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
