FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Smurf, smo
FlyBase ID
FBig0000103355
Interaction Network
Interactions Browser links
Smurf network
smo network
Reported Interactions
FBrf0225546-6.coIP.WB
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Smurf 

Smurf

bait

FLAG tag

smo 

Smo

prey

Myc tag

smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal domain
necessary binding region

"ΔC" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2013)

Smurf interacts with smo but does not affect smo stability.

(Huang et al., 2013)
FBrf0225546-7.coIP.WB
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

Smurf 

Smurf

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Smurf interacts with smo but does not affect smo stability.

(Huang et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2013)
FBrf0238147-1.coIP.WB
Reference
Li et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
smo 
Smurf 

Smurf

prey

FLAG or HA tag

Smurf 
Smurf 
Smurf 
Smurf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
SAID domain
necessary binding region
aa 656-755
smo 
PKA phospho target residues 667,687,740
mutation decreasing interaction
aa 667,687,740

S667D,S687D,S740D

Smurf 
HECT domain
necessary binding region
aa unspecified
Smurf 
HECT domain
sufficient binding region
aa unspecified
Smurf 
Gprk2 phospho target residues 160,164,165,168
mutation decreasing interaction
aa 160,164,165,168

S160A,S164A,S165A,S168A

Smurf 
Gprk2 phospho target residues 160,164,165,168
mutation increasing interaction
aa 160,164,165,168

S160D,S164D,S165D,S168D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment of cells with hh conditioned medium decreased the smo-Smurf interaction. Mutating PKA phospho target residues 667,687,740 in smo from S to A rendered the smo-Smurf interaction refractive to hh signaling. The smo-Smurf interaction also requires Gprk2 activity and regulation of smo-Smurf via Gprk2 requires the N-terminus of Smurf.

(Li et al., 2018)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)