Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Evi5 specifically binds the GTP-bound state of Rab14 and Rab6 (not the GDP-locked forms).

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Evi5 interacts weakly with the GTP-locked form of Rab14, and more strongly with the GTP-locked form of Rab6 (not the GDP-locked forms).

Two-hybrid system: yeast GAL4-BD/GAL4-AD