FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eya, so
FlyBase ID
FBig0000103589
Interaction Network
Interactions Browser links
eya network
so network
Reported Interactions
FBrf0188574-1.coIP.WB
Reference
Mutsuddi et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eya 

EYA

bait

FLAG tag

eya 
so 

SO

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eya 
interaction domain
mutation decreasing interaction
aa 655

S655P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mutsuddi et al., 2005)

Most eya mutations tested do not affect binding to so.

(Mutsuddi et al., 2005)
FBrf0190132-1.Y2H
Reference
Kenyon et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
so 

SO

bait

fused to GAL4 DNA-binding domain

so 
eya 

Eya

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
Six and Homeobox domains
sufficient binding region

"SD+HD" construct, coordinates not reported

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kenyon et al., 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kenyon et al., 2005)
FBrf0190234-2.Y2H
Reference
Kenyon et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
so 

SO

bait

fused to Gal4 DNA binding domain

so 
eya 

Eya

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
SD domain
sufficient binding region
aa 103-213

coordinates relative to so-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast Gal4-BD/GAL4-AD; positive control

(Kenyon et al., 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Kenyon et al., 2005)
FBrf0190234-8.PD.A
Reference
Kenyon et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
so 

SO

bait

GST tag

so 
eya 

Eya

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
SD and HD domains
sufficient binding region
aa 103-213 and 224-274

coordinates relative to so-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kenyon et al., 2005)
FBrf0217457-1.coIP.WB
Reference
Anderson et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
eya 

eya

bait

HA tag

so 

so

prey

Myc tag

so 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
interaction domain
necessary binding region
aa 98-217
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Anderson et al., 2012)
FBrf0231324-1.coIP.WB
Reference
Jin and Mardon, 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
eya 

Eya

bait

FLAG tag

so 

So

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

eya threonine phosphatase activity is not required for its interaction with so.

(Jin and Mardon, 2016)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jin and Mardon, 2016)
FBrf0236135-2.PD.WB
Reference
Davis and Rebay, 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
so 

So

bait

GST tag

eya 

Eya

prey

FLAG tag

eya 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eya 
interaction domain
sufficient binding region
aa 486-760

coordinates relative to eya-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Davis and Rebay, 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)