FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rab7, Lrrk
FlyBase ID
FBig0000103706
Interaction Network
Interactions Browser links
Rab7 network
Lrrk network
Reported Interactions
FBrf0217544-2.coIP.WB
Reference
Dodson et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rab7 

Rab7

bait

GFP tag

Rab7 
Lrrk 

Lrrk

prey

Myc tag

Lrrk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lrrk 
activation loop of kinase domain
mutation decreasing interaction
aa 1914

G1914S

Rab7 
GTPase domain
mutation increasing interaction
aa 22

T22N, dominant negative

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Dodson et al., 2012)

The G1914S mutation of Lrrk reduces binding to Rab7 but not Rab5. Lrrk binds preferentially to the dominant negative form of Rab7 (over the constitutively active form).

(Dodson et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)