FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gyc76C, PlexA
FlyBase ID
FBig0000103812
Interaction Network
Interactions Browser links
Gyc76C network
PlexA network
Reported Interactions
FBrf0223742-1.coIP.WB
Reference
Chak and Kolodkin, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Gyc76C 

Gyc76C

bait

Myc tag

PlexA 

PlexA

prey

HA tag

PlexA 
PlexA 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PlexA 
ectodomain plus transmembrane domain
sufficient binding region
aa 1-1330

coordinates relative to PlexA-PA

PlexA 
endodomain
sufficient binding region
aa 1305-1945

coordinates relative to PlexA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chak and Kolodkin, 2014)

Gyc76C binds the extracellular domain of PlexA more strongly than the intracellular domain.

(Chak and Kolodkin, 2014)
FBrf0223742-3.coIP.WB
Reference
Chak and Kolodkin, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
PlexA 

PlexA

bait

HA tag

Gyc76C 

Gyc76C

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chak and Kolodkin, 2014)
FBrf0223742-4.coIP.WB
Reference
Chak and Kolodkin, 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gyc76C 

Gyc76C

bait

Myc tag

PlexA 

PlexA

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Chak and Kolodkin, 2014)

Transgenic proteins expressed in neurons using elav-GAL4.

(Chak and Kolodkin, 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)