FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tud, aub
FlyBase ID
FBig0000103850
Interaction Network
Interactions Browser links
tud network
aub network
Reported Interactions
FBrf0207339-1.coIP.MS
Reference
Thomson et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

TUD

bait
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Thomson et al., 2008)
FBrf0209532-1.PD.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

biotin tag

aub 
tud 

Tud

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
sufficient binding region
aa 1-25

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide commercially synthesized; prey derived from wild-type ovary extract.

(Nishida et al., 2009)
FBrf0209532-10.CS.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

neutral component
tud 

Tud

neutral component
tud 

Tud

neutral component
aub 

Aub

neutral component
aub 

Aub

neutral component
aub 

Aub

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0209532-2.coIP.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait
tud 

Tud

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)

aub is not sDMA-modified in a csul mutant background.

(Nishida et al., 2009)
FBrf0209532-3.coIP.WB
Reference
Nishida et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

bait
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nishida et al., 2009)
FBrf0209562-1.AF.MS
Reference
Kirino et al., 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

biotin tag

aub 
tud 

Tudor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
sufficient binding region
aa 1-20

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide synthesized in vitro; prey derived from wild-type ovary extract.

(Kirino et al., 2010)
FBrf0209562-2.AF.WB
Reference
Kirino et al., 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

biotin tag

aub 
tud 

Tudor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
sufficient binding region
aa 1-20

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide synthesized in vitro; prey derived from wild-type ovary extract.

(Kirino et al., 2010)
FBrf0209562-3.coIP.WB
Reference
Kirino et al., 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tudor

bait
aub 

Aub

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kirino et al., 2010)
FBrf0209562-4.PD.A
Reference
Kirino et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

FLAG tag

aub 
tud 

Tudor

prey

[35]S label

tud 
tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
mutation disrupting interaction
aa 11,13,15,17

R11K,R13K,R15K,R17K, coordinates relative to aub-PA

tud 
interaction domain
necessary binding region
aa 2148-2515

coordinates relative to tud-PA

tud 
interaction domain
sufficient binding region
aa 1-300,1545-2515

coordinates relative to tud-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait immunopurified from transfected S2 cells; prey produced and labeled by in vitro translation.

(Kirino et al., 2010)
FBrf0211676-1.SPR
Reference
Liu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

peptide, symmetrically dimethylated at R11, R13 or R15

aub 
tud 

Tud7-11

prey
tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
interaction domain
sufficient binding region
aa 2344-2515

coordinates relative to tud-PA

aub 
interaction domain
sufficient binding region
aa 6-18

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant protein in bacterial system; peptide commercially synthesized.

(Liu et al., 2010)

In this assay, tud binds the R15 dimethylated form of aub most strongly and the R11 dimethylated form most weakly.

(Liu et al., 2010)
FBrf0211676-2.XC
Reference
Liu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

eTud11

neutral component
tud 

eTud11

neutral component
tud 
tud 

eTud11

neutral component
tud 
aub 

Aub

neutral component

peptide, symmetrically dimethylated at R13 or R15

aub 
aub 

Aub

neutral component

peptide, symmetrically dimethylated at R13 or R15

aub 

Aub

neutral component

peptide, symmetrically dimethylated at R13 or R15

aub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
interaction domain
sufficient binding region
aa 2344-2515

coordinates relative to tud-PA

aub 
interaction domain
sufficient binding region
aa 6-18

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant protein in bacterial system; peptide commercially synthesized.

(Liu et al., 2010)
FBrf0211676-3.ITC
Reference
Liu et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

eTud11

bait
tud 
tud 
aub 

Aub

prey

peptide, symmetrically dimethylated at R11, R13 or R15

aub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
interaction domain
sufficient binding region
aa 2344-2515

coordinates relative to tud-PA

tud 
interaction domain
mutation decreasing interaction
aa 2432

N2432A, coordinates relative to tud-PA

aub 
interaction domain
sufficient binding region
aa 6-18

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant protein in bacterial system; prey peptide commercially synthesized.

(Liu et al., 2010)

Kd of aubR15(me2s)-tud interaction = 5.4E10-6M, Kd of aubR13(me2s)-tud interaction = 4.8E10-5M, Kd of aubR11(me2s)-tud interaction = 7.1E10-5M, Kd of aubR13(me2s)-tud,N2432A mutant interaction = 1.6E10-3M

(Liu et al., 2010)
FBrf0212296-1.coIP.WB
Reference
Creed et al., 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

aub

bait
tud 

tud

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Creed et al., 2010)
FBrf0212296-2.coIP.WB
Reference
Creed et al., 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

aub

bait

GFP tag

tud 

tud

prey

HA tag

tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
tud domains 2-6
mutation decreasing interaction
aa 301-1544
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins immunopurified from tagged transgenic constructs from ovaries of transgenic fly line.

(Creed et al., 2010)
FBrf0229343-8.coIP.WB
Reference
Sato et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Tud

bait
tud 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

tud still interacts with aub in krimp mutant flies.

(Sato et al., 2015)

Source was ovaries of Oregon-R fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sato et al., 2015)
FBrf0237152-2.coIP.WB
Reference
DeHaan et al., 2017
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

GFP tag

tud 

Tud

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from tagged transgenic construct and purified from ovaries; prey produced as a recombinant fusion protein in baculovirus and Sf9 cell system.

(DeHaan et al., 2017)
FBrf0244439-3.PD.WB
Reference
Vo et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

GFP tag

tud 

Tud

prey

His tag

tud 
tud 
tud 
tud 
tud 
tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
Tud domain 1
sufficient binding region
aa 1-217

coordinates relative to tud-PA

tud 
Tud domain 3
sufficient binding region
aa 592-772

coordinates relative to tud-PA

tud 
Tud domain 4
sufficient binding region
aa 1002-1192

coordinates relative to tud-PA

tud 
Tud domain 6
sufficient binding region
aa 1299-1484

coordinates relative to tud-PA

tud 
Tud domain 9
sufficient binding region
aa 1976-2160

coordinates relative to tud-PA

tud 
Tud domain 11
sufficient binding region
aa 2335-2515

coordinates relative to tud-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait purified from transgenic ovary extract; prey produced as a recombinant fusion protein in bacterial system.

(Vo et al., 2019)
FBrf0249362-8.coIP.WB
Reference
Huang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

GFP tag

aub 
aub 
tud 

Tud

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
methylation sites
mutation disrupting interaction
aa 11,13,15,17,26

R11K,R13K,R15K,R17K,R26K, coordinates relative to aub-PA

aub 
piRNA binding domain
mutation disrupting interaction
aa 568,572

Y568L,K572E, coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Huang et al., 2021)
FBrf0259220-2.PD.WB
Reference
Wahiduzzaman et al., 2024
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

GFP tag

tud 

Tud

prey

HA tag

tud 
tud 
tud 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
tudor domain 1-2
sufficient binding region
aa 1-585
tud 
tudor domain 8-9
sufficient binding region
aa 1791-2160
tud 
tudor domain 10-11
sufficient binding region
aa 2140-2515
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and purified from tagged transgenic construct in ovary extract.; prey produced as a recombinant fusion protein in bacterial system.

(Wahiduzzaman et al., 2024)
FBrf0259220-5.PD.WB
Reference
Wahiduzzaman et al., 2024
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tud 

Tud

bait

HA tag

tud 
aub 

Aub

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tud 
tudor domain 7-11
sufficient binding region
aa 1605-2515
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Pyk competes with aub for binding to tud domains 7-11.

(Wahiduzzaman et al., 2024)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and purified from tagged transgenic construct in ovary extract.

(Wahiduzzaman et al., 2024)
FBrf0262483-2.CL.FM
Reference
Salgania et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
tud 

Tudor

bait

mCherry tag, fused to the plasma membrane

aub 

Vasa

prey

EGFP tag

aub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
symmetric dimethylated arginine residues
mutation disrupting interaction
aa 11, 13, 15, 17

R11K/R13K/R15K/R17K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

tud was fused to a plasma membrane anchor. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction. Mutation of aub arginines rendered them unmodifiable by arginine methyltransferases and disrupted aub-tud interaction.

(Salgania et al., 2024)

Source was live S2R+ cells; bait and prey produced from tagged transgenic construct.

(Salgania et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (11)