FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gyc76C, kermit
FlyBase ID
FBig0000103948
Interaction Network
Interactions Browser links
Gyc76C network
kermit network
Reported Interactions
FBrf0223742-5.Y2H
Reference
Chak and Kolodkin, 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gyc76C 

Gyc76C

bait

fused to LexA DNA-binding domain

Gyc76C 
Gyc76C 
kermit 

dGIPC

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyc76C 
C-terminal region
sufficient binding region
aa 1451-1525

"KC1" construct

Gyc76C 
C-terminal region
mutation disrupting interaction
aa 1523-1525

"KC2" construct, lacks terminal 3 residues

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Chak and Kolodkin, 2014)

Two-hybrid system: yeast LexA-BD/B42-AD

(Chak and Kolodkin, 2014)
FBrf0223742-6.coIP.WB
Reference
Chak and Kolodkin, 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Gyc76C 

Gyc76C

bait

Myc tag

Gyc76C 
Gyc76C 
kermit 

dGIPC

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyc76C 
C-terminal region
necessary binding region
aa 1105-1525

"Δ(C+P)" construct

Gyc76C 
C-terminal region
mutation decreasing interaction
aa 1523-1525

"ΔPBM" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chak and Kolodkin, 2014)

Overexpression of kermit increases cell surface localization of Gyc76C.

(Chak and Kolodkin, 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)