FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
teq, orb2
FlyBase ID
FBig0000104001
Interaction Network
Interactions Browser links
teq network
orb2 network
Reported Interactions
FBrf0211209-2.PD.WB
Reference
Mastushita-Sakai et al., 2010
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
teq 

Teq

bait

biotin label

teq 
teq 
teq 
orb2 

Orb2

prey

HA tag

orb2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
teq 
3-prime UTR
sufficient binding region
unspecified
teq 
3-prime UTR
mutation decreasing interaction
UUUUGU

UUUUGU to UUCAGU

orb2 
Zn-finger domain
mutation disrupting interaction
aa 640-704

deletion, coordinates relative to orb2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type or transgenic adult head extract.

(Mastushita-Sakai et al., 2010)
FBrf0217407-8.PD.WB
Reference
Majumdar et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
teq 

Tequila

bait

biotin label

orb2 

Orb2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro translation; prey derived from S2 cell extract.

(Majumdar et al., 2012)
FBrf0230300-1.PD.WB
Reference
Khan et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
teq 

Tequila

bait

biotin label

teq 
teq 
teq 
orb2 

Orb2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
teq 
3-prime UTR
sufficient binding region
teq 
primary Orb2 recruitment site M2
necessary binding region
UUUUGU

"ΔM2" construct

teq 
Orb2 recruitment site M1
necessary binding region
UCCACCU

"ΔM1" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from transfected S2 cell extract.

(Khan et al., 2015)

Reporter assays indicate that monomeric orb2 represses teq translation and inhibits recruitment to polysomes, while amyloidogenic orb2 oligomer increases recruitment of teq to polysomes.

(Khan et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)