FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
brat, nanos
FlyBase ID
FBig0000104003
Interaction Network
Interactions Browser links
brat network
nanos network
Reported Interactions
FBrf0135777-4.Y2H
Reference
Sonoda and Wharton, 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

Nos

bait

fused to GAL4 DNA-binding domain

nanos 
brat 

Brat

prey

fused to GAL4 activation domain

brat 
brat 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
C-terminal region
sufficient binding region
aa 288-401

coordinates relative to nos-PA

brat 
NHL domain
sufficient binding region
aa 736-1037

coordinates relative to brat-PA

brat 
NHL domain residue G774
sufficient binding region
aa 774

G774D, coordinates relative to brat-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sonoda and Wharton, 2001)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Sonoda and Wharton, 2001)
FBrf0247403-11.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

Myc tag

nanos 

Nos

prey
nanos 
nanos 
nanos 
nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR proximal
sufficient binding region
nt 21-100
nanos 
3-prime UTR miRNA binding sites
mutation disrupting interaction
nt 34-40,76-81

GGCAGCU to GGUAGUU and CAUUCC to CAUUUU

nanos 
3-prime UTR distal
sufficient binding region
nt 781-851
nanos 
3-prime UTR Sxl binding site
mutation disrupting interaction
nt 787-800

UUUUUUUAUUUUUU to UUUCUCUAUCUCUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Malik et al., 2020)
FBrf0247403-3.coIP.PCR
Reference
Malik et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

Myc tag

nanos 

Nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Malik et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)