FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cno, Ran
FlyBase ID
FBig0000104004
Interaction Network
Interactions Browser links
cno network
Ran network
Reported Interactions
FBrf0216492-2.PD.A
Reference
Wee et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cno 

Cno

bait

GST tag

cno 
cno 
Ran 

RanGTP

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cno 
RA1 domain
sufficient binding region
cno 
RA2 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ful length cno, cno RA domain 1 and cno RA domain 2 preferentially bind to Ran GTP over Ran GDP or Ran loaded with the GTP analogue GMPPNP

(Wee et al., 2011)

Interaction in vitro; bait and prey produced as a recombinant fusion protein in bacterial system.

(Wee et al., 2011)
FBrf0216492-3.coIP.WB
Reference
Wee et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
cno 

Cno

bait

GFP tag

Ran 

Ran

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wee et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)