Source was embryonic cytoplasmic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
piwi protein was purified by sequential DEAE sepharose, BioRex-70, Heparin and Superdex 200 column chromatography to isolate associated factors.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced by in vitro translation; prey produced by in vitro translation.