FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ctp, spn-F
FlyBase ID
FBig0000104127
Interaction Network
Interactions Browser links
ctp network
spn-F network
Reported Interactions
FBrf0228877-3.coIP.WB
Reference
Otani et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ctp 

ctp/LC8

bait

Myc tag

spn-F 

Spn-F

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Otani et al., 2015)
FBrf0228877-7.coIP.WB
Reference
Otani et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spn-F 

Spn-F

bait

GFP tag

spn-F 
spn-F 
ctp 

ctp/LC8

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spn-F 
coiled-coil region 2
sufficient binding region
aa 191-273

"C1" construct, coordinates relative to spn-F-PA

spn-F 
ctp-interacting region
necessary binding region
aa 230-275

"Δ231" and "Δ253" constructs, coordinates relative to spn-F-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Otani et al., 2015)
FBrf0230080-2.coIP.WB
Reference
Lin et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ctp 

Ctp

bait

FLAG

spn-F 

SpnF

prey

myc

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lin et al., 2015)

Addition of IKKε with mutation G250D increases the strength of the interaction.

(Lin et al., 2015)
FBrf0230080-5.coIP.WB
Reference
Lin et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ctp 

Ctp

bait

FLAG

spn-F 

SpnF

prey

myc

spn-F 
spn-F 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spn-F 
CC3 domain
mutation disrupting interaction
aa 210-241
spn-F 
SCD domain
mutation disrupting interaction
aa 313-339
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lin et al., 2015)

Removal of the either the CC3 or SCD domain from spn-F disrupts the interaction.

(Lin et al., 2015)
FBrf0246588-3.coIP.MS
Reference
Lu et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spn-F 

Spn-F

bait

GFP tag

ctp 

ctp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Lu et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)