Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Chromatin was digested to primarily mono- and dinucleosomes with micrococcal nuclease prior to immunoprecipitation.

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Chromatin was digested to primarily mono- and dinucleosomes with micrococcal nuclease prior to immunoprecipitation.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

The molecular weight of a Cenp-C-cal1 complex estimated by SEC-MALS is consistent with 1cal1 :2Cenp-C stoichiometry.

The ability of Cenp-C fused to LacI to recruit cal1 to LacO arrays was taken as evidence of a Cenp-C-cal1 interaction.

Source was U2OS cells; bait produced from transfected construct; prey produced from transfected construct.

The ability of cal1 fused to LacI to recruit Cenp-C to LacO arrays was taken as evidence of a Cenp-C-cal1 interaction. Co-expression of cid strongly enhances the Cenp-C-cal1 interaction.

Source was U2OS cells; bait produced from transfected construct; prey produced from transfected construct.

Bait protein was fused to lacI, which targets the chimera to transgenic chromosomal lacI binding sites. The ability of this chimera to recruit prey protein to the lacI binding sites was taken as evidence of direct physical interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Cosedimentation analysis is consistent with a stoichiometry of 2 Cenp-C : 1 cal1.