FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
scrib, Vang
FlyBase ID
FBig0000104215
Interaction Network
Interactions Browser links
scrib network
Vang network
Reported Interactions
FBrf0208559-2.Y2H
Reference
Courbard et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Stbm

bait

fused to GAL4 DNA-binding domain

Vang 
scrib 

Scrib

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
C terminal domain
sufficient binding region
aa 294-584
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Courbard et al., 2009)

l(2)gl, baz, par-6, Patj, and sdt did not interact with Vang in this experiment.

(Courbard et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Courbard et al., 2009)
FBrf0208559-3.PD.A
Reference
Courbard et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Stbm

bait

GST tag

Vang 
scrib 

Scrib

prey

[35]S label

scrib 
scrib 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
C terminal domain
sufficient binding region
aa 294-584
scrib 
PDZ-3 domain
sufficient binding region
aa 1151-1234
scrib 
PDZ-4 domain
sufficient binding region
aa 1264-1333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Courbard et al., 2009)

The PDZ-3 domain of scrib showed a much stronger interaction with Vang as compared to the PDZ-4 domain.

(Courbard et al., 2009)
FBrf0208559-4.PD.A
Reference
Courbard et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
scrib 

Scrib

bait

GST tag

scrib 
Vang 

Stbm

prey

[35]S label

Vang 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
C terminal domain
sufficient binding region
aa 294-584
scrib 
PDZ3-4 domain
sufficient binding region
aa 1151-1333
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Courbard et al., 2009)
FBrf0257118-4.coIP.WB
Reference
Humphries et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

FLAG tag

scrib 

Scrib

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

positive control

(Humphries et al., 2023)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Humphries et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)